In the Perry lab, we are fueled by a desire to make a difference in understanding and intervening in disease states in which metabolism is relevant – and by the fact that doing science is fun. We work in complementary in vivo and in vitro rodent systems. Most projects, though led by an individual, are collaborative – we all (including the PI) participate hands-on to some extent in most projects. We are committed to fostering an enrichment that values diversity and inclusion.
Several sub-projects are available within each of these themes. Please click on project titles to be taken to a list of relevant literature (from our lab and others).
Obesity is associated with increased incidence and poorer prognosis of over a dozen tumor types, but the mechanism for this is not well understood. We use unique tracer methods to examine substrate utilization in tumors and determine both how tumor metabolism is altered with obesity, and how this modulates cell division.
We are combining our experience in both muscle physiology and tumor metabolism to explore how exercise slows tumor growth, with the goal to identify the molecule(s) and/or mitochondrial flux(es) responsible for the anti tumor effect of exercise.
Cancer immunometabolism is a hot topic of great clinical interest. We are interested in exploring how the immune cell fuels its functions, and whether we can module anti-cancer immune activity using metabolically-based therapies.
During certain stress states (e.g. infection, anaphylaxis, severe hypoglycemia, and others), the body must reallocate substrates. Evolution has tuned the body to do this exquisitely, but human understanding has not caught up. We are exploring this phenomenon mechanistically, aiming to identify crucial factors for substrate partitioning, and to understand the changes in tissue-specific fluxes that they cause. This is a close collaboration with Dr. Andrew Wang’s lab.
In order to better understand the intersection between metabolism and survival under (patho)physiological conditions, we are developing, validating, and utilizing methods to explore substrate metabolism in tumor, kidney, and others.
Metabolic rate per gram tissue scales inversely with body size: if a human’s metabolism were as fast as a mouse’s, that human would need to consume 35,000 calories a day to maintain body weight. We want to understand why.
This list is not exhaustive; we add new ones all the time!
- Mouse care and handling
- In vivo infusions and metabolic studies in mice
- Liquid chromatography/mass spectrometry
- Nuclear magnetic resonance spectroscopy
- Oxidative metabolism using the Alabama Tissue Slicer
- Biochemical analysis of glucose and lipid metabolites (ELISA, mass spec, enzymatic assays)
- Cell culture and in vitro metabolic assays
We hold combined meetings with the labs of Drs. Gerald Shulman and Richard Kibbey on Wednesdays at 3pm in TAC S247. Visitors are welcome, but please contact Rachel beforehand – mostly to make sure lab meeting is on for the week(s) you want to attend.